BUCHI detectors and any detector equipped with an analog output 0-1V can be connected with the Sepacore® X50 system.
To connect the ELSD to the Sepacore® X50 system you need the flow splitter kit. The flow splitter kit is designed to provide a small flow rate to the ELSD C-650 (< 5ml/min) and is compatible with an inlet flow rate up to 250 mL/min.
The biggest column which can be used is a BUCHI glass column 920 mm x 100 mm. Such a column can be packed with about 3700 g silica (40 – 63 µm). This means than the maximum sample load can be higher than 300 g (depending on type of sample).
It allows running reversed phase separations
even at high flow rates. The solvents used in
reversed phase applications (such as water /
MeOH) have a higher viscosity than the ones
used for normal phase separations: in particular
the mixtures of water and methanol have
a viscosity twice as large as water alone. This
is the reason why only a medium pressure
pump can be used for high scale reversed
Such a medium pressure is also useful to
work with smaller particle size columns: BUCHI
Silica HP is 15μm instead of 40 - 63μm
for other standard packing materials and the
back pressure generated significantly grows
when the particle size decreases: Back pressure
= k / (particle size)2.